WebApr 8, 2024 · Affimer proteins represent a group of alternative binding proteins that can be used to study protein expression and function. The image shows an antibody (Blue … WebJun 3, 2024 · The use of Affimers as detection molecules also provides additional benefits due to their low production cost and consistent batch uniformity. In our hands, these tests …
Introduction and Key Feature Comparison - Avacta Life …
WebAug 9, 2024 · They also discuss the ease with which Affimers can be adapted for different applications; for example, Affimers that are coupled to GFP via rigid linkers are suitable for fluorescence polarisation microscopy, and have been used to investigate the orientation of F-actin filaments in live cells. Combining Affimers with super-resolution imaging WebFeb 23, 2024 · The binding efficiency of these Affimers to the CEA receptor was thoroughly studied and confirmed by using surface plasmon resonance as reported by Shamsuddin et al. The surface plasmon resonance showed that the Affimers demonstrated a high binding affinity toward CEA (K D value for clone II: 15.3 ± 0.37 nM; K D value for clone III: 34.4 ± … atara wyatt
Isolation of isoform-specific binding proteins (Affimers) by …
WebNov 14, 2024 · They can also be used as research and affinity reagents, for diagnostic purposes, and as therapeutics. However, the ability to identify isoform-specific reagents remains highly challenging. We describe an adapted phage display protocol using an artificial binding protein (Affimer) for the selection of isoform-selective binding proteins. WebIn order to test the specificity and sensitivity, two different labeling systems were used; biotin and creating an Affimer-alkaline phosphatase fusion protein. Our current data … WebJun 27, 2024 · Here we report on the use of Affimer proteins as research reagents. We selected 12 diverse molecular targets for Affimer selection to exemplify their use in common molecular and cellular applications including the (a) selection against various target molecules; (b) modulation of protein function in vitro and in vivo; (c) labelling of tumour ... asimo at disneyland