Chromotek gfp-trap agarose
WebJun 14, 2024 · This protocol describes the immunoprecipitation with GFP-Trap® Agarose (product code: gta). GFP-Trap® Magnetic Agarose (product code: gtma) or GFP-Trap® … WebBoth the agarose and magnetic bead formats are extremely efficient for immunoprecipitation of GFP tagged proteins, and the usual heavy and …
Chromotek gfp-trap agarose
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WebGFP-PFKFB3 was overexpressed in HEK293T cells and immunoprecipitated using GFP-Trap agarose beads. The PFKFB3 complex was then incubated with the indicated amount of recombinant human Src protein for 10 min. Western blot was used to detect tyrosine-phosphorylation level. WebGet more from your GFP/RFP fusion. Alpaca GFP-Trap® is the top selling GFP antibody as it allows for one-step pulldowns that can be directly used in downstream applicaitons …
WebJan 10, 2024 · The mixture was then incubated with the GFP-Trap Agarose beads (Chromotek, pre-washed by 1 mL extraction buffer) for 4 h at 4 °C. Then, the beads were collected by spinning and washed with the extraction buffer for 3–5 times to eliminate nonspecific bindings. The bound proteins on the GFP-trap were boiled and assayed by … WebProduct Information. GFP-Trap® Agarose is an affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads. Description. …
WebYou can use Chromotek GFP-trap magnetic agarose beads for the pull-down/co-immunoprecipitation assays. These magnetic beads are coupled to GFP nanobody and are ready to use. Only you need to label ... WebDec 16, 2024 · ChromoTek's Nano-Traps are optimized for the immunoprecipitation (IP) of proteins and their interacting factors. Nano-Traps comprise of a Nanobody/ V H H conjugated to beads. For 3 Nano-Traps, e.g. GFP-Trap ® , RFP-Trap ®, and Spot-Trap ®, we offer different types of beads: Agarose beads Magnetic Agarose beads Magnetic …
WebThe ChromoTek GFP-Trap® Agarose Kit consists of an anti-Green Fluorescent Protein (GFP) Nanobody (VHH), which is covalently bound to agarose beads. GFP-Trap Agarose Kit is used to immunoprecipitate GFP-fusion proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc.
WebApr 11, 2024 · For example, we noted this caveat when purifying TDP-43 aggregates with a 434 Chromotek nanobody, GFP-trap magnetic agarose (#gta-10) instead of 10782-2-AP or 3H8. In this 435 scenario, we found that the nanobody was destabilized by the 1% Tween-20-containing extraction buffer 436 Jo urn al Pre- p oo f and resulted in virtually no yield … dick helton knx radioWebApr 5, 2024 · Bus, drive • 46h 40m. Take the bus from Miami to Houston. Take the bus from Houston Bus Station to Dallas Bus Station. Take the bus from Dallas Bus Station to … citizenship id bhutanWebIt natively comes with conventional UT, TOFD and all beam-forming phased array UT techniques for single-beam and multi-group inspection and its 3-encoded axis … citizenship immigration status i-9 formWebApr 11, 2024 · A 500 μl fraction of each cleared sample was mixed with 50 μl of pre-equilibrated GFP-Trap magnetic agarose (ChromoTek Cat No. gtma) or DYKDDDDK Fab-Trap Agarose (ChromoTek Cat No. ffa) and incubated over-night at 4°C rotating end-over-end. The beads were then washed twice with Wash buffer (10 mM Tris–HCl pH 7.5; 150 … dick henry auctionsWebAlpaca GFP-Trap® is the top selling GFP antibody as it allows for one-step pulldowns that can be directly used in downstream applicaitons such as enzyme activity assays or mass spectrometry. 2. Best results, qualitative and quantitative GFP-/RFP trap are so easier to use than any other pull down reagent. dickheiser ethnicityWebThe ChromoTek GFP-Trap™ Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. Brand: Proteintech GTMA-400 Additional Details : Weight : 0.00480kg View more versions of this product Product Code. 17353363 55828.28 NOK / 400 reactions Quantity Estimated Shipment: 12-05-2024 Add to basket Description Specifications dick helton knx retiringWebChromoTek GmbH. F2H®-Kit Basic: Kit comprises F2H®-BHK Cell Line, 1 vial 5 X 106 frozen cells (f2h-bhk); 100 µg Platform Reagent (f2h-p100); and 30 µg Control-DNA (f2h-c30) Platform Reagent: Transfection supplement enabling assembly of the nuclear PPI-platform to anchor GFP-tagged proteins; for 125 transfections (24-well format) or 500 ... citizenship immigration status options