WebElevated O-GlcNAcylation level promoted NLRX1 ubiquitination and decreased NLRX1 stability proved by ubiquitination and cycloheximide (CHX) chase experiments, and enhanced the interaction between NLRX1 and inhibitor of nuclear factor kappaB kinase-α (IKK-α), thus reducing the expression of inflammatory cytokine IL-1β in M1 macrophages. WebApr 10, 2024 · A cycloheximide (CHX) pulse-chase experiment was then performed to confirm that OTUD6B stabilizes the β-TrCP protein. To do so, β -TrCP with wild-type OTUD6B or OTUD6B C188S were co-transfected into HEK293T cells, and the stability of β -TrCP was found to be prolonged in the presence of wild-type OTUD6B but not OTUD6B …
Quantitative Cell-based Protein Degradation Assays to …
WebJul 5, 2024 · All the experiments are representatives of three independent biological experiments with similar results. Dengue Virus (DENV) Capsid Protein Is Degraded in an Autophagy-Dependent Manner ... (CHX) in DENV-infected cells after Baf A1 and 3-MA treatments. CHX chase assay showed that both Baf A1 and 3-MA treatments could … WebSep 11, 2024 · SUM159 cells in the CHX chase experiment as presented in Figure 1A. Western blot images were quantified using the ImageJ software. (B) Normalized intensity of SNAI2 protein with or without MG132 treatment, as presented in Figure 1A. Data is presented as mean ± SEM. n = 3. **p<0.01 by Student’s t-test. (C) Quantification of low lying pregnancy bump
QPCT could bind with HRAS and promote the stability of
WebJun 15, 2024 · However, the CHX-chase experiments showed that rotenone-induced Ser129-phosphorylated α-syn was targeted to the proteasome pathway at the same rate as normally phosphorylated α-syn. It should be noted that in the present experimental condition, ATP-dependent proteasome activity was not lost by mitochondrial impairment. ... http://www.protocol-online.org/biology-forums-2/posts/22692.html WebJan 1, 2015 · 100 mg/ml cycloheximide (CHX) stock solution (Sigma-Aldrich, catalog number: C7698) ... For an unfamiliar protein, it is recommended to start with a 4-hour … low lying right cerebellar tonsil