Chx10-cre

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August undefined, 2024

WebFeb 24, 2024 · Chx10 Antibody (D-11) is a mouse monoclonal IgG 2b κ, cited in 12 publications, provided at 200 µg/ml. specific for an epitope mapping between amino … WebJul 15, 2004 · To further understand Chx10 regulation and function, a multifunctional reporter construct consisting of GFP, alkaline phosphatase, and Cre recombinase was …

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WebJul 30, 2008 · In contrast, the previously characterized, proximal Chx10 CRE is sufficient for AP reporter expression in bipolar cells and progenitor cells in transgenic mice (Rowan and Cepko, 2005). The role of the distal 164 bp Chx10 CRE might thus be to increase Chx10 transcription at a time when bipolar cells first appear and/or thereafter. In this regard ... WebDec 15, 2008 · The Chx10-Cre mouse line contains a random integration of the BAC-Chx10-Cre transgene. This transgene includes a fusion gene of Cre and GFP, and an … how to season old cast iron

Chx10 Cre Mouse Line The Jackson Laboratory Bioz

WebTrying to get openVPN to run on Ubuntu 22.10. The RUN file from Pia with their own client cuts out my steam downloads completely and I would like to use the native tools already … WebChx10Cre; Rblox/loxp107−/−; Diclox/+ retinoblastoma tumors retain a wild-type and functional Dic allele. (a) Schematic representation of the Dic wild-type, floxed and Cre … WebMay 1, 2024 · For Chx10-Cre, Vglut3-Cre and control C57BL/6J mice, AAV8-Syn-DIO-hM4Di-mCherry (4.6–6.7 × 10 12 vg/ml; UNC Vector Core) was injected into the cervical spinal cords of P14 mice at 4 locations all within levels C4–7 (0.8 μL / location, 0.4 mm lateral, 0.5 mm depth for Chx10-Cre and control, 0.4 mm depth for Vglut3-Cre, 0.6–0.8 … how to season or prepare sweet potatoes

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WebMar 9, 2024 · Transcriptome analysis of Chx10-Cre Rb1/p107 and Chx10-Cre Rb1/p107/Hells P21 retinae: GSE144428: Chromatin accessibility analysis of Chx10-Cre Rb1/p107 and Chx10-Cre Rb1/p107/Hells P21 retinae: Relations: BioProject: PRJNA603598: Download family: Format: SOFT formatted family file(s) SOFT: WebFigure 1. Targeting strategy for the generation of conditional Ret-knockout mice and genotyping of C-Ret lx/lx and Chx10-Cre;C-Ret lx/lx mice using polymerase chain reaction (PCR). The Cre-loxP system was used to generate conditional Ret-knockout mice.In C-Ret lx/lx mice, the target, exon 12, of the C-Ret gene is flanked by two loxP sites (A, top).The … how to season oven cooked turkeyWebJul 4, 2012 · Generation of retina-specific Th knock-out mice. Chx10-Cre mice purchased from The Jackson Laboratories were crossed with conditional Th mice to obtain Th lox/lox mice that carry no copies or a … how to season orange roughy fish

"WebJun 18, 2024 · Three cohorts of mice were studied: retinal-specific Bmal1 knockout mice ( Chx10cre − Bmal1fl/fl; hereafter referred to as “ rBmal1 KO” mice) and two control groups: 1) Bmal1fl/fl mice (i.e., littermates of the rBmal1 KO mice expressing only Bmal1fl/fl ); and 2) Chx10cre mice (carrying the eye-specific Chx10-CRE transgene, and to which the … " - Chx10-cre

Chx10-cre

Dual requirement for Pax6 in retinal progenitor cells

WebTherefore, the Cre-GFP fusion protein appeared to be labile in retinal cells that had exited the cell cycle. (Fig. 1P). Furthermore, expression levels of all three Hh signaling effectors, Gli1 ... Web005105 STOCK Tg(Chx10-EGFP/cre,-ALPP)2Clc/J These Chx10 BAC mice express a transgene containing EGFP and human placental alkaline phosphatase with reporter …

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WebNov 5, 2024 · Chx10 is expressed in retinal progenitor cells during retina genesis and is gradually restricted to bipolar cells in adult mice []. Chx10-cre was known to be …

WebJul 27, 2024 · Transgenic mice expressing Cre recombinase with the Chx10 promoter (Chx10-Cre mice 17) were mated with Hif-1αflox/flox mice 18 to obtain sensory-retina-specific Hif-1α knockout mice. Hif-1αflox/flox mice without the Cre transgene were used as control mice. The genetic background of all transgenic mice was C57BL/6J. WebFigure Legend Snippet: Chx10-Cre Lsd1 fl / fl showed modest signs of retinal thinning and irregular morphology in Hematoxylin and Eosin (H&E) staining compared to controls; …

WebThe 8th codon of Chx10 is joined in-frame to an EGFP-cre fusion gene followed by an internal ribosome entry sequence (IRES) coupled to the human placental alkaline … WebMar 9, 2024 · Conversely, CHX10-Cre might not knockout Bmal1 in RPE cells, because CHX10 is a transcriptional factor localized to the inner nuclear layer, particularly in …

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WebC, the plexus regions in Chx10-Cre;Rb Lox/À ; p107 À/À tumors (shown at three increasing magnifications) were filled with synaptic densities among adjoining neurites that were associated with ... how to season pampered chef bakewareWebApr 14, 2024 · Recently Concluded Data & Programmatic Insider Summit March 22 - 25, 2024, Scottsdale Digital OOH Insider Summit February 19 - 22, 2024, La Jolla how to season palayokWebThe Cre-mediated transgene expression in retinal bipolar cells in the Chx10-cre line was not observed by crossing with the reporter mouse line but through Cre-dependent rAAV vector delivery. how to season pampered chef cast ironWebNov 5, 2024 · Chx10 is expressed in retinal progenitor cells during retina genesis and is gradually restricted to bipolar cells in adult mice []. Chx10-cre was known to be expressed in bipolar and a significant number of Müller glial cells [9, 32].To determine cells with Chx10-cre expression in this study, we used Rosa26-tdTomato reporter mice.Red fluorescent … how to season oyster crackersWebSep 24, 2024 · They were mated to Chx10-cre mice and backcrossed into the BALB/cJ background for four generations to change strains, and then mated to produce mice of the following genotypes: Chx10-cre +;AMPKα1 fl/fl or Chx10-cre +;AMPKα2 fl/fl. All mice were reared in 12-h light–12-h dark cycles (lights on at 6 AM) with 60-lx lighting intensities at … how to season oven roasted potatoesWebAdult male Chx10 Cre/+ mice (lacking the ROSA PNP- (Gi)DREADD/+ allele) were used as non-DREADD expressing controls. On rare occasions, we observe recombination outside of the nervous system as a result of “leaky” germline or early embryo expression of … how to season panWebMutation details: A BAC containing the Chx10 locus was modified by insertion of a cre recombinase-polyA sequence into the first exon of Chx10. Line 5 was generated. Line 5 … how to season peas