Cytochrome c aptamer
WebJun 24, 2015 · Cytochrome c (Cyt c) is a heme-containing metalloprotein located in the intermembrane space of mitochondria. It plays a central role in electron transport chain and it is also an intermediate in apoptosis. When mitochondria are injured under pathological conditions, Cyt c is released into the cytosol of the cell. WebDec 17, 2024 · Cytochrome c (Cyt c) is a heme protein (about 12 kDa molecular weight) that plays a role in essential biological functions such as electron transport, metal-ion …
Cytochrome c aptamer
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WebCytochrome c (Cyt c ), as an important biomarker, plays an important role in cell apoptosis and the mitochondrial electron transport chain. Here, we have developed a novel biosensor for a Cyt c assay based on polydopamine nanotubes (PDANTs) and an exonuclease I (Exo I) cyclic amplification strategy. WebApr 23, 2002 · DNA aptamers were selected for their ability to bind simultaneously to the protein cytochrome c and to the metalloporphyrin hemin. Such aptamers each …
WebOct 26, 2024 · Aptamer-functionalized QDs can detect and monitor the presence of analytes by observing changes in the PL of the QDs. For example, an Mn-ZnS QD biosensor has been developed to detect cytochrome c (Cyt c) [ 44, 45 ]. Mn doping can enhance the optical and electronic transport of QDs [ 61, 62 ]. WebMay 1, 2024 · Cytochrome C, tris (2,2′-bipyridyl)-dichlororuthenium (II) hexahydrate (Ru (bpy)3 Cl 2 ·6H 2O), tripropylamine (TPA), ferrocene carboxylic acid (Fc-COOH), 1-Ethyl …
WebApr 12, 2024 · This method exhibited significant selectivity against target among ten coexisting proteins (fibrinogen, hemoglobin, cytochrome C, serum albumin, alkaline … Webaptamer-mediated phosphorescent sensor with high specificity and operational simplicity can effectively avoid the interference of scattering light from complex substrates. Our findings offer a new clue for building biosensors based on QDs and aptamers. 1. Introduction Cytochrome c (Cyt c) is a hemachrome-containing metal micromolecular ...
WebJan 15, 2024 · Herein, a novel upconversion@polydopamine core@shell nanoparticle (termed as UCNP@PDA NP) -based aptameric biosensor has been fabricated for the quantitative analysis of cytochrome c (Cyt c) inside living cells, which comprises an UCNP@PDA NP, acting as an internal reference and fluorescence quenching agent, and …
WebApr 5, 2024 · A method is introduced for the fluorometric turn-on detection of cytochrome C (cyt c) by using a specific aptamer and nanosheets composed of fluorescent graphitic carbon nitride (g-C3N4) nanosheets. … how many people use buses in indiaWebApr 1, 2011 · This work characterized the binding of an RNA aptamer recognizing hen egg white lysozyme, as well as a literature-reported single-stranded DNA analog of sequence identical to the original RNA aptamer, using fluorescence anisotropy, isothermal titration calorimetry (ITC) and analytical ultracentrifugation. how many people use buffWebJun 18, 2024 · MF-3WJ consisted of three arms, namely, an IFN-γ aptamer sequence, a thiol group, and a C–C mismatch sequence. Ag + was inserted between the C–C mismatched sequences to stabilize the double strand, and Ag + was used as a redox label . The reliability of the electrochemical biosensor was secured by measuring seven … how can you improve your skillsWebFeb 1, 2014 · In this work, we present a label -free impedimetric aptasensor for the recognition of cytochrome c (Cyt c) at a pM concentration level based on an epoxy-graphite composite electrode.The technique employed for the detection of the protein Cyt c is electrochemical impedance spectroscopy (EIS). Detection occurs when the protein … how many people use cheggWebNov 16, 2024 · The ssDNAs, including the random libraries used for SELEX, the anti-IgE aptamer, as well as those found binding to DNMT1 (Supplementary Tables S1 and S3) were attained from Integrated DNA Technologies, Inc. Beta-casein, cytochrome C, ovalbumin, and hemoglobin were purchased from Sigma-Aldrich. IgE was acquired from … how many people use cannabisWebThe nanocontainer was fabricated by immobilizing cytochrome c (CytC) onto the MSNs as sealing agent via intermediate linkers of disulfide bonds for redox-responsive intracellular drug delivery ... how many people use buy now pay laterWebDec 17, 2024 · Cytochrome c (Cyt c), a heme protein, can be a potential biomarker for cell-apoptosis or even cancer diagnosis. In this work, a simple, rapid, sensitive and selective label-free assay for... how many people use cable