Tīmeklis© 1987 Nature Publishing Group #. !! .E -o "' ·-3: ., ~ E b (% . .,,7 of = •
A combinatorial enhancer recognized by Mad, TCF and Brinker …
Approximately 500 μl volume of 0–6 h-old embryos were homogenized in lysis buffer (50 mM Tris–HCl (pH 7.5), 150 mM NaCl, 2.5 mM EDTA, 0.2% Triton X, 5% glycerol, complete EDTA-free protease inhibitor cocktail (Roche)) and spun at 500gfor 5 mins at 4 °C. The supernatants obtained were made up to 1 ml with … Skatīt vairāk The following stocks were used: hs-FLP hs-I-SceI (BL6935), ey-FLP (BL5580), w1118 (BL5905), Gal4::VP16-nos.UTR (BL7293), c355 … Skatīt vairāk The trk-null mutant was generated using ends-out gene replacement33. First, the donor plasmid pW25-trk was built by cloning 2.7 Kbp of upstream (F- 5′-AGCAGGCGCGCCTTCAGTGTCATTGCGAAAGATT … Skatīt vairāk Adults were allowed to lay on media containing apple juice supplemented with yeast paste for 24 h before being removed. … Skatīt vairāk T7 RNA polymerase (Roche) was used to transcribe a 980 bp fragment of tll (F-5′-AGTGCTTTGAGGTCGGAATG-3′, R-5′-TTGAGACCTTGTGCATCAGC-3′) that had been cloned into pGEM-T easy (Promega) to … Skatīt vairāk TīmeklisCo-knockdown of Fur1 and Fur2 results in terminal defects including a reduced posterior filzkorper (fz, arrowed) and head skeleton defects (arrowheads) similar to embryos … outboard motors for sale in alaska
(PDF) Supplementary Information - ResearchGate
Tīmeklis2001. gada 1. okt. · Gregory Ponomareff a , Heidi Giordano c , Yvonne DeLotto b , and Robert DeLotto b a Cornell Graduate School of Medicine, New York, New York 10021, b Department of Genetics, University of Copenhagen, Copenhagen K, Denmark c Tularik, South San Francisco, California 94080 Corresponding author: Robert DeLotto, … Tīmeklis2012. gada 28. jūn. · (D) faf F08 /faf B4 cuticle displays significant ventralization with a herniated head, defective Filzkorper, malformed dorsal cuticle and denticle belts that extend well into the dorsal half of ... Tīmeklis2007. gada 24. okt. · Filzkorper defects in dpp and brk mutant embryos suggest that two rounds of Dpp signaling occur during posterior spiracle development. High … roll brim hat pattern