Phellsgate 12
WebpHELLSGATE 12 High-throughput binary Gateway® vector with two introns, for silencing genes in plants using intron-containing hairpin RNA. Open in SnapGene Try SnapGene for … WebSep 14, 2024 · For Sl-AGL11 down-regulation, a construct enabling RNAi silencing was designed using the pHELLSGATE-12 system (Invitrogen). The Sl-AGL11 3ʹ end was …
Phellsgate 12
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WebAug 1, 2003 · The pHELLSGATE vectors contain convenient restriction sites that can be used to check for the presence of inserted sequences and diagnose the orientation of the … WebDec 23, 2001 · The pHELLSGATE vector was tested using 200 and 400 nt PCR products for two different genes. Many bacterial colonies were obtained on chloramphenicol …
Webhomology to any other tomato gene was amplified and cloned into pHELLSGATE 12 vector (Figure S2). A total of ten independent transgenic lines bearing MSH2-RNAi construct were raised using Agrobacterium-mediated transformation. Southern blot analysis of T 0 lines revealed the presence of the transgene infive lines (Figure S3). In T WebNov 23, 2011 · A schematic map of the Gateway cloning system-based RNAi vector, pSGate1. The ccdB cassettes were derived from pHellsgate-12 ( 24) and cloned into the silencing vector pSilent1 ( 9 ). Unique restriction sites are indicated.
WebpHELLSGATE 12 Vector Database Welcome to Vector Database! Vector database is a digital collection of vector backbones assembled from publications and commercially available … Web2 baths, 1638 sq. ft. house located at 17312 Bishopsgate Dr, Pflugerville, TX 78660 sold for $110,026 on Sep 23, 1998. View sales history, tax history, home value estimates, and …
WebOne of the pH12-PpeIFiso4G11 transformed plants is resistant to PPV-M and PPV-D infection Transgenic Japanese plum lines were initially inoculated with the PPV-M20 isolate (which belongs to the PPV-M strain) as described in Decroocq et al [25].
WebDan Hernandez October 12, 2011. There's a Bank of America ATM just outside the gate. 13 Photos. Related Searches. gate a12 philadelphia • gate a12 philadelphia photos • gate a12 … harmish guitarsWebpHELLSGATE_12_1x[1].png. 102.83 KB; Cite. 1 Recommendation. 2nd Nov, 2015. Mehreen Zeb. Riphah International University. you can use the softwares NEB cutter or restriction … chanting baboon persona 5 weaknessWebPhellsgate 2 Vector Thermo Fisher Bioz Bioz Stars score, Techniques, Protocol Conditions and more for Phellsgate 2 Vector, supplied by Thermo Fisher. Data for Phellsgate 2 … chanting bird and flowing fountainWebMar 20, 2024 · The SHPRH (SNF2, histone linker, PHD, RING, helicase) subfamily belonging to ATP-dependent chromatin remodeling factor is the effective tumor-suppressor, which can polyubiquitinate PCNA (proliferating cell nuclear antigen) and participate in post-replication repair in human. However, little is known about the functions of SHPRH proteins in plants. harmis freundinWebAlternatively, generic gene-silencing vectors such as the pHANNIBAL and the pHELLSGATE series can be used. They simply require the insertion of PCR products, derived from the target gene, into the vectors by conventional cloning or by using the Gateway directed recombination system. chanting aumWebIn this paper we describe two improved pHellsgate vectors that facilitate rapid generation of hpRNA-encoding constructs. pHellsgate 4 allows the production of an hpRNA construct in … harmire road barnard castle dl12 8dtWebPMID: 17147629 DOI: 10.1111/j.1467-7652.2005.00149.x Abstract We describe here a vector system that allows dexamethasone-inducible RNAi against plant genes. The system utilizes a modified pHELLSGATE vector, under the control of the pOp6 promoter, and the synthetic transcription factor, LhGR. harmisons fundraising.com