Phis1522
Webb1 feb. 2013 · Results: Using GFP, α-amylase and TcdA-GT as model proteins, high level of intracellular protein expression (up to 250 mg/L for the GFP) was achieved in Brevibacillus, using the pHis1522 vector carrying the B. megaterium xylose-inducible promoter (PxylA). WebbEventually, mutations were introduced in pHIS1522/TcdB by site-directed mutagenesis (QuikChange, Stratagene, La Jolla, CA, USA). Cloning of TcdB constructs with internal deletions was performed in the pHIS1522 vector backbone by first introducing a silent SpeI restriction site at bp position 2542 of the TcdB sequence with the QuikChange method.
Phis1522
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WebbTaKaRa brevibacillus competent cells takara bio Brevibacillus Competent Cells Takara Bio, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. WebbJuly 13, 2015: Primers were ordered for the pHis1522-aTcdB and pHsp70-Cas9 vectors. The primers were designed to install 21 extra bp at the ends, to be used as homologous …
WebbP1522 OBD Code Definition: P1522 A Camshaft Position Actuator Bank 2 P1522 OBD Code Description: Possible Symptoms Malfunction Indicator Light (MIL) ON Possible Causes … Webb27 feb. 2024 · IPTG act as a molecular mimic of allolactose and initiate transcription of lac operon and trigger expression of the inserted genes under the control of this Lac operon (in this case HriGFP gene). The HriGFP pet28+ construct was directly transformed in E. coli (BL21DE3 cells), however, for transformation in Bacillus megaterium the gene was sub ...
WebbThere are wide range of programs have been written to perform primer selection, varying significantly in selection criteria, comprehensiveness, interactive design, and Webb6 nov. 2008 · Background Major Clostridium difficile virulence factors are the exotoxins TcdA and TcdB. Due to the large size and poor stability of the proteins, the active recombinant TcdA and TcdB have been difficult to produce. Results The toxin genes tcdA and tcdB were amplified by PCR using chromosomal DNA from a toxigenic strain as a …
WebbJuly 2, 2015: An aliquot of purified pHis1522-aTcdB vector was acquired from Dr. Xingmin Sun from the Tufts Vet School. NIH-recommended simple letter format MTA forms were signed by both parties. (aTcdB = atoxic Clostridium difficile toxin B) July 6, 2015: The pHis1522-aTcdB was diluted 1:10 and 1ul was transformed into JM109 E. coli.
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