WebbpHis2载体价格1000元,pHis2载体质粒图谱(Vector map),载体序列(Sequence)见下文,载体质粒抗性为Kanamycin,质粒大小为7207bp。pHis2是酵母单杂交载体的pHis2。 http://www.youbio.cn/product/vt1637
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WebbY187-pHis2 Yeast One-Hybrid Media Kit(酵母单杂培养基套装YM1010),含有用于Y187-pHis2系统酵母单杂交文库筛选的全套培养基,性价比更高。. 培养基以非常方便使用的铝箔袋形式包装,每个独立包装可以配制0.5 L的培养基。. 使用时仅需要将袋中的所有干粉倒入 … Webb服务时间:24小时在线客服 . 客服热线:400-000-7085 how to split oak
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Webb挑取pHIS2的单个克隆接种于含有Kan(50 mg/L)的LB液体培养基中于37℃摇床中180 r/min过夜培养,用质粒小量提取试剂盒提取质粒后用Eco R Ⅰ和Sac Ⅰ进行双酶切,并纯化回收。 1.2.3 连接及转化. 将稀释后的复性双链DNA片段与切后的pHIS2进行连接,反应体系如 … Webb载体描述. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One- Hybrid Library Construction & Screening Kit (#K1617-1). It contains a HIS3 nutritional reporter gene, located downstream of a multiple ... WebbpHIS2 can be maintained in both yeast and bacteria. It contains an autonomous replication se-quence ( ARS4) and TRP1 nutritional marker for replication and selection in yeast (1, … reach ++ meaning