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Phis2自激活

WebbpHis2载体价格1000元,pHis2载体质粒图谱(Vector map),载体序列(Sequence)见下文,载体质粒抗性为Kanamycin,质粒大小为7207bp。pHis2是酵母单杂交载体的pHis2。 http://www.youbio.cn/product/vt1637

酵母单杂交详细实验流程-钟鼎生物

WebbY187-pHis2 Yeast One-Hybrid Media Kit(酵母单杂培养基套装YM1010),含有用于Y187-pHis2系统酵母单杂交文库筛选的全套培养基,性价比更高。. 培养基以非常方便使用的铝箔袋形式包装,每个独立包装可以配制0.5 L的培养基。. 使用时仅需要将袋中的所有干粉倒入 … Webb服务时间:24小时在线客服 . 客服热线:400-000-7085 how to split oak https://americanffc.org

上海宝吉生物-酵母文库构建,酵母文库筛选,酵母双杂交文库构建 …

Webb挑取pHIS2的单个克隆接种于含有Kan(50 mg/L)的LB液体培养基中于37℃摇床中180 r/min过夜培养,用质粒小量提取试剂盒提取质粒后用Eco R Ⅰ和Sac Ⅰ进行双酶切,并纯化回收。 1.2.3 连接及转化. 将稀释后的复性双链DNA片段与切后的pHIS2进行连接,反应体系如 … Webb载体描述. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One- Hybrid Library Construction & Screening Kit (#K1617-1). It contains a HIS3 nutritional reporter gene, located downstream of a multiple ... WebbpHIS2 can be maintained in both yeast and bacteria. It contains an autonomous replication se-quence ( ARS4) and TRP1 nutritional marker for replication and selection in yeast (1, … reach ++ meaning

pHis2_载体及菌株库_产品中心_奥诺基因-HonorGene-基因研究资 …

Category:酵母表达载体构件 – 聚生物

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Phis2自激活

知无不“研” 一文读懂酵母单/双杂交点对点验证 - 知乎

Webb24 apr. 2016 · Figure 酵母单杂和双杂筛选的一般步骤,更加详细单杂和双杂筛选流程图,请参考Figures BDMatchmaker TM 单杂和双杂文库筛选与构建. 如这个图表所示,As diagramshows,重组介导克隆使文库构建和筛选更快捷高效, 尽管这里没有显示,双杂文库能通过酵母融合筛选(细节 ... Webb6 sep. 2024 · 1)pHis2-bait和AD-prey质粒混合作为实验组,将pHis2-bait和空载AD质粒混合作为自激活组。 将实验组和自激活组分别共转到Y187感受态细胞中。 2)用SD/-Leu/ …

Phis2自激活

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Webb1、pHis2-Bait或pAbAi-Bait和AD-prey质粒 2、或者Bait和Prey序列,质粒由我们构建 1、提供详细的、完整的实验报告,清晰的实验图片 2、在受体菌的阳性菌 自激活和毒性验证: … WebbPhis2 1 Bait Plasmid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results > TaKaRa > phis2. plasmid phis2 1 TaKaRa is a verified supplier TaKaRa manufactures this product . About; News ...

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WebbpHIS2 PVT4037 2ug Lot no 1901093 Store at -20℃ Expiry date June.2024 pHIS2 Information Promoters: TRP1, T7, T3 Replicon: pUC ori, FI ori Plasmid classification: … how to split obs audioWebb本文针对酵母单杂技术的基本原理、基础知识、主要用途做了简要介绍;并以Y1Hgold(pAbAi-bait)和Y187(pHis2-bait)为例,介绍了自激活检测的目的以及主要 … how to split objects in sketchupWebb30 nov. 2024 · 技术原理:2个结构域彼此分离但为功能必需的结构域,单独作用不能激活转录反应,当二者在空间上充分接近时,则呈现完整的GAL4转录因子活性使启动子下游基因(抗性筛选、蓝白斑筛选、营养缺陷型筛选等)得到转录。 把需要检测的蛋白质分别与BD和AD融合,形成 bait 融合蛋白(BD- bait)和 prey 融合蛋白(AD-prey),如果 bait … reach 01743 664063http://www.biogenesci.com/ reach + careers portalreach 0.1%Webb26 maj 2024 · pHIS2 can be maintained in both yeast and bacteria. It contains an autonomous replication sequence (ARS4) and TRP1 nutritional marker for replication … reach 1 foundationhttp://www.genecreate.cn/preferential_news/2444.html how to split local disk in windows 10