Phis2.1 vector

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August undefined, 2024

Webb16 okt. 2014 · Specifically, the bait construct pHis2.1−2 × 150 was generated by cloning tandem copies of the 150-bp enhancer (2 × 150) into the MluI site of pHis2.1, a one … Webb24 jan. 2024 · For ligation: the molar ratio of your bait sequence: vector ranges from 3:1 and 5:1. The reaction can be put at 16 degrees overnight (in PCR cycler machine). It …

Matchmaker One-Hybrid Library Construction & Screening Kit

Webb使用方法: 1.计算所需平板的数量，计算配置培养基体积；. 2.设置3-AT的浓度梯度（建议10-50 mM 的浓度范围设3-5个梯度，常用浓度约30 mM）；. 3.灭菌筛选培养基，待培养基冷 … Webb一、载体概念. 1. 载体，DNA的搬运工. 载体：在基因工程重组DNA技术中，将DNA片段（目的基因）转移至受体细胞的一种能自我复制的DNA分子。. 2. 载体，基因密码承载者. 载 … how many ferraris have been sold

Transcription Factor-Centered Yeast One-Hybrid Assay

Webb载体描述. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use … Webb5 juli 2024 · The empty vector pHIS2.1 was used as the negative control. All cells carrying different vector combinations grew normally and equally on the SD/-Leu/-Trp medium (Fig. 4 b). However, the growth of yeast cells with the combination of pGADT7/pHIS2.1 empty vectors or pGADT7- GsERF71 /pHIS2.1 vectors was significantly inhibited on the SD/ … Webb19 sep. 2024 · The pHIS2 vector carries the TRP1 nutritional marker for selection in yeast. The transformed yeast cells were cultured on SD/-Trp-Leu medium and SD/-Trp-His-Leu medium supplemented with 10 mM or 30 mM 3-amino … how many feral cats are euthanized

(PDF) Exploring transcriptional signalling mediated by OsWRKY13, …

http://www.biofeng.com/zaiti/jiaomuzajiao/pHis2.html Webb5 apr. 2024 · In the present study, we identified 14 L. kaempferi ARFs(LkARFs), and found LkARF7 and LkARF19 bound to LkBBM1promoter and enhanced its transcription using yeast one-hybrid, ChIP-qPCR, and dual-luciferase assays. In addition, the treatment with naphthalene acetic acid promoted the expression of LkARF7and LkARF19. how many ferraris were used in magnum piWebb29 okt. 2024 · DNA-protein interactions were verified by growth performance of yeast cells co-transformed with the indicated pairs of Rec2 and pHis2.1 vectors on synthetic defined (SD) medium –Leu–Trp–His containing 3-amino-1,2,4-triazole (3-AT). how many ferrari f40 were made

"Webb25 okt. 2016 · In the mutated assay, the sequence of the G-box in the NWRE (GCGTTCTTACTTTTCGTAAGGG CACGTG AAGCACCCCAACAAA) was replaced with … " - Phis2.1 vector

Phis2.1 vector

Identification and characterization of the novel Col10a1 regulatory ...

WebbCoconut (Cocos nucifera L.) is a member starting the palm tree family (Arecaceae) and the only living species of the genus Cocos. In this paper, the regulatory relationships directions between more transcription influencing and functional genes inhered identified due combining ATAC-seq and RNA-seq in … Webb方法通过克隆 MaAQP1 的启动子，将启动子构建到pHIS2诱饵质粒上，转化酵母菌构建诱饵表达载体，同时构建干旱胁迫的香蕉（ Musa acuminat L. AAA group cv. Brazilian）cDNA文库。结果克隆获得1 362 bp的启动子序列，通过分析其顺式作用元件，结果显示启动子序列中共有72个顺式作用元件，包括了TATA-box和CAAT-box核心元件，ABA响应元件 …

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WebbA yeast one-hybrid library was constructed to screen the binding proteins of suspensor specific cis-element. In this study, five repeats of suspensor specific cis-element … WebbIn this construct, three copies of the DNA target (T) have been inserted into the pHIS2.1 reporter vector. GAL UAS minimal promoterADE2, HIS3, lacZ, and MEL1 transcription …

WebbpTALEN v2 (NN) pTALEN v2 (HD) pX335 pX260 pX320 pShuttle-CMV pShuttle pAdTrack-CMV pAdTrack pAdEasy-1 BJ5183 AdEasy-1 pAd/BLOCK-iT-DEST RNAi Gateway Vector pDC315 pBHGloxdelE13cre pShuttle-IRES-hrGFP2 pAAV-MCS pAAV-RC2 pAAV-RC pHelper pAAV-LacZ pAAV-IRES-hrGFP pCMV-MCS RNAi-Ready pSIREN-RetroQ RNAi-Ready … http://www.labbase.net/Supply/SupplyItems-4229347.html

WebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … Webb27 mars 2024 · 筛选含有报道质粒的酵母细胞具体过程为：合成靶序列；将靶序列插入pHIS2载体的多克隆位点；一般为将0.1mg pHIS2质粒用EcoRⅠ和SacⅠ进行完全双酶 …

WebbUnique restriction sites are in bold. pHIS2.1 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the Matchmaker™ One-Hybrid Library Construction & Screening Kit (Cat. No. 630304).

Webb9 apr. 2024 · The recombinant vectors were transferred into yeast strain Y187 cells using the LiAC-PEG4000 method. The TF-encoding genes were amplified and inserted into the pGADT7 vector, and the resulting constructs were then transformed into the pHis2 yeast strain containing the promoter plasmid. high waisted light wash blue jeans levihttp://www.honorgene.com/uploads/files/202411/6181dfe1813d2.pdf high waisted light pink lingerieWebb26 mars 2024 · The promoter sequence (1800 bp) of MdMATE8 (MDP0000175055) was inserted into the pHIS2.1 vector (Clontech, Palo Alto, CA, United States), which … high waisted light wash flare jeanshttp://www.biofeng.com/zaiti/jiaomu/pYES2.html high waisted light wash denim jeanshttp://www.youbio.cn/product/vt1637 high waisted lilac bikiniWebb24 aug. 2024 · The promoter of each gene was cloned and inserted into the pHis2.1 vector to generate reporter constructs. TcMYC2 was fused to the GAL4 activation domain to … how many ferries drba hasWebbpHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the … high waisted lightweight cropped gym leggings