Phospho-specific antibodies
WebPhospho-specific antibodies are used not only to detect phosphorylated proteins or peptides, but also to measure changes in their level and specificity of modification by … WebPhospho-Specific Antibodies For analyzing cell signaling pathway activation Our phospho-specific antibody range References For Analyzing Cell Signaling Pathway Activation View …
Phospho-specific antibodies
Did you know?
WebThis will provide you with absolute confidence in the specificity of your phospho-specific antibody and determine if expression of the target protein is up- or downregulated (Figure 1) Loading controls: popular loading controls include housekeeping proteins, such as beta-actin or beta-tubulin. WebIP. Phospho-Rpb1 CTD antibody specificity is demonstrated using peptide dot blot. Antibody binding to pre-coated Rpb1 CTD peptides is shown using Phospho-Rpb1 CTD (Ser2) …
WebMonoclonal Antibody for studying Bad (Ser99) phosphate. Cited in 1 publication. Highly specific and rigorously validated in-house, Phospho-Bad (Ser136) (5D8) Mouse … Webprotein, phosphosite-specific antibodies recognize the phospho-amino acid but within the context of the amino acids surrounding the phosphorylation site. In other words, the binding of any given phosphosite-specific antibody encompasses stretches upstream and downstream of the phosphorylation site. The value of these reagents
WebThe process of producing antibodies against a phospho-residue is more complicated than traditional antibody production using peptide immunogens. Phospho-specific antibodies are generated using peptides containing one or more phosphorylated amino acids. There are three residues which can be phosphorylated: Serine (S), Threonine (T) and Tyrosine (Y). WebWhen selecting a phospho-specific antibody for your experiments, it is crucial to ensure that the antibody specifically detects the protein of interest only when it is phosphorylated at …
WebThe antibody was cross adsorbed to unphosphorylated AIFM1 (Ser-116) peptide before affinity purification using phospho-AIFM1 (Ser-116) peptide. This antibody antibody detects a triplet at 66, 62, and 57 kDa* protein on SDS-PAGE immunoblots of human Jurkat cells treated with calyculin A.
WebImmunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ABL1/ABL2 (phospho Y393/429) polyclonal antibody (Cat # PAB12190). Immunofluorescence; Immunofluorescence staining of methanol-fixed HeLa cells using ABL1/ABL2 (phospho Y393/429) polyclonal antibody (Cat # PAB12190, red). small estate affidavit laws in ohioWebJun 5, 2015 · The eight phospho-specific antibodies were chosen to represent major signaling nodes responsive to cytokine, TLR, and antigen receptor signaling. This antibody panel is used with 8 standard stimulation conditions (unstimulated, IFNa, IL-6, IL-7, IL-10, IL-21, LPS, PMA+ ionomycin), although other stimuli can be added. small estate affidavit nj form freeWebApr 9, 2024 · “A Pan-specific Antibody for Direct Detection of Protein Histidine Phosphorylation” The idea is that the antibody is: ‘pan’ in that it will react with all phospho-histidine residues on proteins, but ‘specific’ in that it will not react with unphosphorylated histidine residues or other phospho-aminoacids e.g. phospho-serine. small estate affidavit mohave county arizonaWebPhospho-specific antibodies Antibodies that discriminate between the phosphorylated and the non-phosphorylated state of a protein, is one of the most frequently asked for types of … small estate affidavit parker county texasWebPolyclonal Antibody for studying POLR2A (Ser1616) phosphate. Cited in 3 publications. Validated for Western Blotting, Immunoprecipitation. Highly specific and rigorously validated in-house, Phospho-Rpb1 CTD (Ser2) Antibody (CST #8798) is ready to ship. small estate affidavit in njWebPhospho-specific antibodies are primary antibodies that detect only the phosphorylated forms of proteins and recognize the phosphorylated serine, threonine, or tyrosine residues in the context of the rest of the protein. small estate affidavit orange county cahttp://www.innovagen.com/phospho-specific-antibodies song if i was a carpenter and you were a lady