WebNov 4, 2016 · 将PCR反应管放入Thermo 9700型PCR扩增仪, 选择预设PCR扩增程序, 开始运行反应。 PCR反应程序为: 94℃预变性2分钟; 30个循环: 94℃变性30秒, 58℃退火30秒, 72℃延伸30秒; 最后72℃延伸5分钟。 WebMar 7, 2024 · Genome-wide identification of PME family genes from Citrus sinensis. To identify PME family genes from C. sinensis, HMMER and BLASTP analyses of the sweet orange genome (C. sinensis, version 2) were performed [].The predicted CsPME proteins containing both PME and PMEI domains were designated as Type-1 PMEs, and the …
史上最全限制性内切酶酶切位点汇总 - 360文档中心
WebPmeI–NEB酶试剂 ; OXOID培养基 CM0003B 营养琼脂 Nutrient Agar 500G/瓶 ... T090RS-Q-Thermo QSP盒装收尖吸头 黄色 无菌 ... WebPmeI We are excited to announce that all reaction buffers are now BSA-free. NEB began switching our BSA-containing reaction buffers in April 2024 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. Find more details at www.neb.com/BSA-free. Isoschizomers Single Letter … how to check switch port speed
MssI (PmeI) (5 U/µL) - Thermo Fisher
WebNEB’s restriction enzyme buffer system makes your restriction digests easy and convenient. We are able to offer >210 restriction enzymes that cut in a single buffer, rCutSmart™ . This improves ease-of-use, especially when performing double digests. In addition to indicating the performance of each enzyme in the 4 NEBuffers, the chart also ... WebMar 31, 2024 · The PmeI (Thermo Sci-entific) digested vector w as then used together with the three . digested fosmids f or TAR cloning in yeast. Y east colonies . WebProduct Source. An E. coli strain that carries the cloned FseI gene from Frankia species Eul1b (NRRL 18528). This product is related to the following categories: Restriction Endonucleases C G, Time-Saver Qualified Restriction Enzymes Products. This product can be used in the following applications: Fast Cloning: Accelerate your cloning ... how to check sync between domain controllers