WebBioAssay Systems’ sialic acid assay uses a single Working Reagent that combines NANA aldolase, pyruvate oxidase and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to sialic acid concentration in the sample. WebSep 8, 2024 · The specific recognition of ybbR sequence (DSLEFIASKLA) by Sfp synthetase is used to realize the specific labeling of influenza virus NA protein and M2 protein. Labeling of NA can be used to study the virus shedding process. NA releases the virus from host cells by cleaving sialic acid on the host cell surface.
Clontech Labs 3P SIALIC ACID FLUORESCENCE LABEL
WebThe Sialic Acid (GlyS) Kit provides a rapid and convenient method for relative screening of terminal sialic acid content in crude or purified samples. Sialic acid data can be combined … WebThis is in contrast to the research conducted on the SLC35A1 (CMP–sialic acid transporter) knockout, for which such an effect was observed (31. ... Figure 7 Metabolic labeling of cellular N-glycans with radioactive ... The fluorescence signal from the 2-AB-labeled N-glycans (black solid lines) was overlaid with the radioactivity data (red ... how do you know if your obese
Workflow Ordering Guide - Sialic Acid Analyis of Biotherapeutic ...
WebMar 12, 2010 · Acids with lower pK a values, such as citric acid, malic acid, and malonic acid, were shown to result in higher yields than acetic acid when the derivatization was performed at 37 °C for 16 h. When higher temperatures and very acidic conditions are applied, partial degradation of the glycans will be induced with loss of sialic acids [ 49 ]. WebTaKaRa sialic acid fluorescence labeling kit Sialic Acid Fluorescence Labeling Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more WebThe relative amounts of sialic acid on the surface of the three cell lines were determined by FITC-SNA, a specific lectin that binds to a-2,6 linked sialic acid. The a-2,6 linked sialic acid content in leukema cells (K562/MDR, and Jurkat cells) was significantly higher than that of HEK293 cells ( Figure 3D ). phone call scams 2020